The 30-year-old striker was taken off at half-time during a 2-0 friendly win over Wales for 'precautionary' reasons, later dismissing speculation that it was because of a groin injury.
"I wouldn't call it an injury. It built up during the game so I decided it was sensible to come off", said van Persie, who scored in his country's previous two friendies against Ecuador and Ghana.
Van Persie was out of action for six weeks last season with a knee injury, still ending with 18 goals in his 28 showings for Manchester United in 2013/14.
The Netherlands claimed a comfortable victory over Wales in their most recent friendly, a first half goal by Arjen Robben and another by substitute Jeremain Lens later in the game wrapping up to match for the Dutch.
Manager Louis van Gaal - who will join van Persie at Manchester United following the World Cup - said: "He has a little injury and I think it is better that you don't risk a bigger injury playing the second half.
"I think he made the right decision. It is not an important match. You don't have to risk it."
The Netherlands finished runners-up in the 2010 World Cup, and will face champions Spain on 13 June in their first game of the 2014 tournament.
The Dutch and Spanish sides will meet in Group B alongside Chile and Australia, and you can compare the World Cup betting odds of all four teams here.
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