Rooney assisted England's only goal against Italy last Saturday but seemed to struggle in his wider role during the 2-1 defeat, sparking debate over Roy Hodgson's choice to play Sterling in Rooney's usual central role.
Liverpool midfielder Sterling insisted however that he would switch roles with the Manchester United striker should it make the difference England need to keep their World Cup hopes alive.
Sterling said: "I would be happy to play anywhere the manager puts me. I will be working for the team regardless of the position I play in.
"It was a big occasion, my first competitive start", the 19-year-old added. "I tried to think of it as just another game and give it my best shot. It wasn't enough but the team did some really impressive stuff and we will go out on Thursday ready to go again.
"I tried to work hard for the team and help not just in the attacking bits but in defence as well. It was a difficult one because they had about three players around me every time."
Rooney himself took to Facebook to vent his frustrations over criticism of his performance against Italy, saying: “Sometimes wonder what the press are getting at.
“I said from the start I want to do everything I can to make sure I’m ready for these World Cup games and as part of that I was doing extra training a week before the squad joined up. That’s exactly what I did, my own extra training because that’s what I wanted to do.”
Uruguay lie bottom of Group D meanwhile after losing 3-1 to Costa Rica last Saturday, but striker Luis Suarez is likely to start this time alongside Edison Cavani. Suarez, the Premier League's top scorer of 2013/14, is 6/1 to net first against England with Bet365.
England take on Uruguay at 8pm in Sao Paulo and the match will be shown live on ITV1. Click here to compare betting odds for England in the 2014 World Cup
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