Netherlands midfielder Rafael van der Vaart will miss the 2014 World Cup after suffering a calf injury during a training session.
The latest in a series of injury problems for 31-year-old former Tottenham midfielder this season means he will not be in Holland coach Louis van Gaal's squad for the tournament, trimmed to 29 provisional players before the cut to 23 occurs for the 2 June deadline.
Van der Vaart would have been the most experienced international player within van Gaal's provisional squad, holding a total of 109 international caps for Holland and having competed in the previous two World Cup encounters in 2006 and 2010.
The provisional Netherlands squad will however see Robin van Persie making the trip, despite the striker having only started one game for Manchester United after sustaining a knee injury in March.
The 2010 World Cup runners up will select their final squad by Monday, which provisionally features a total of seven Premier League players on the 29-man list including van Persie (Manchester United), Swansea's Michel Vorm and Jonathan de Guzman, Aston Villa defender Ron Vlaar, Norwich midfielder Leroy Fer, Newcastle goalkeeper Tim Krul and Manchester City's Karim Rekik - on loan at PSV Eindhoven.
Arjen Robben (Bayern Munich), Wesley Sneijder (Galatasaray), Klaas-Jan Huntelaar (Schalke 04) and Dirk Kuyt (Fenerbahce) also feature as high-profile players under Van Gaal's squad heading the 2014 World Cup, which begins on 12 June.
The Netherlands face the tough task of Group B, squared up with Spain, Chile and Australia which begins with a rematch against reigning champions Spain on 13 June. Compare Netherlands' betting odds for the 2014 World Cup here.